examination of blood in forensic science ppt

Moreover, each microcrystal test binds differently with these 2 active sites. https://doi.org/10.1016/j.forsciint.2013.02.012, Kunz S, Brandtner H, Meyer H (2015) Characteristics of backspatter on the firearm and shooting hand-an experimental analysis of close-range gunshots. Sci Justice 36(3):153160. This results in a procedure that is both more ecient and accurate. Required fields are marked *. Spectroscopic examination of blood (or precisely hemoglobin and its derivative) is considered to be one of the most reliable confirmatory tests for both fresh and dried bloodstains. Textile fibres are one of the most important forms of trace evidence in the context of criminal evidence. Did the stain patterns come from the victim or someone else? There are basically seven major types of confirmatory tests that can be used for the confirmation of whether the possible bloodstain is actually blood or not? If it is five feet above the convergence, the victim may have been standing. 2011). However, when the bloodstain dries, intact RBCs are no longer visible. Microscopic Examination of Paint The microscope is the most important instrument for locating and comparing paint specimens . Certain bands are formed representing different types of Hb. drugs!andother!toxicants!inbloodmay!be!useful!for!establishing!recent! Buffer ph: Hemolysate should be run either of the electrophoresis at a pH of 8.6. Blood is one of the most common pieces of evidence encountered at the crime scene. DNA samples4. 2015). In the case of multiple victims, analysts will often use DNA profiling to determine whose blood is included in a given pattern, helping to estimate the locations of the victims in relation to each other and the perpetrator(s). It involves creation of fake bloodstains using Awlata dye to determine this relation. Singh, P., Gupta, N. & Rathi, R. Blood pattern analysisa review and new findings. Ninety-degree angle was maintained and the Awlata dye was dropped from different heights and observations were made. Using Awlata dye for studying bloodstains can solve these problems because of its easy availability, low cost, and it can be made under laboratory conditions. Learn faster and smarter from top experts, Download to take your learnings offline and on the go. 1. ). And based on the respective opposite charge, they moved to their respective electrodes. There are two different types of blood that can be collected at a crime scene: liquid and dried blood. But as per forensic importance, separating Hemoglobin from the sample is a clear sign that the crime scene sample has blood as a component. Additionally, scientists use blood-typing to determine paternity. Google Scholar, Bear J (1975) Dynamics of fluids in porous media. Video and sketches of the scene and the blood stains is often used to provide perspective and further documentation. a. DNA testing has an unmatched degree of accuracy. In order to determine the donors of different kind of blood spatter throughout the crime scene, methods of DNA analysis should be applied. This lead to separation based on different types of hemoglobins in the form of bands. nearly no false-positive result. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. 3. Procedure Take about 0.1 mg of blood in a test tube. In another study, the velocities of blood were considered with factors like air drag and gravity which was used to predict the back-spatter formation by carrying the experiment using a blood-soaked sponge (Comiskey et al. Forensic Science is the application of science to criminal and civil laws, mainly on the criminal side during criminal investigation, as governed by the legal standards of admissible evidence and criminal procedure. The following is the procedure for the Starch gel electrophoresis. Electrophoresis is carried out at a constant voltage of 150 V (approx) at a pH of 8.6. 2016). Take the overnight frozen sample and place it over the supporting agar gel medium. Once the separation is done, hemoglobin fractions are visualized by staining with leuco malachite green solution. If stain is on cloth, soak the cloth sample in normal saline or Viberts fluid for at least 6 hours. To carry studies to understand bloodstains is not always possible; it requires a large amount of blood which is subjected to ethical clearance. Eligibility of articles was finalized by analyzing whether the papers were discussing about BPA and its related methodology or not. Options and Suggestions for Your Final Exam, a special, last minute Forensic Teacher Supplement, a Bloodspatter Quiz and Forensic Higher Ed Programs, The History of the Secret Service, Forensic Crossword Puzzle, a Mini Mystery, Forensic News, Just For Fun, The Morgue Guy, Stoopid Criminals . These are a set of spectral lines extended to the full spectrum that are used as the metric to categorize the different absorbed spectra. We've encountered a problem, please try again. 2005). 2011). This analysis can be done using strings and a protractor, mathematical calculations or computer models. After the electrophoresis is done, the anti-human serum is placed through the length of electrophoretic migration. Dilute the blood sample with normal saline. Historically, the Federal Bureau of Investigation is often considered the 'go-to' resource for forensic science information and knowledge. Highly characteristically to blood detection. Two similar bands are of Oxyhemoglobin with two extra bands. History and Development of Forensic Science Blood pattern analysis (BPA), i.e., the study of shape, size, and nature of bloodstain. The residual material is allowed to dry on the slide so that it gets fixed. The most frequently used method of capturing bloodstains is high-resolution photography. If the sample isnt completely dry when it needs to be packaged, the sample should be rolled in paper and labeled and then put into either a brown paper bag or a box. And in general scenarios, it is always done after the presumptive test for blood was performed. Eyewitness reliability10. So, with RNA-based assays, the serologist can then depict the origin of the fluid whether it is saliva, blood, semen, and/or their combination. ABSTRACT Forensic science or forensics is the scientific processing of information found at the crime scene. Blood ranges from 45 L (female) to 56 L (male). Identification has, as its purpose, the determination of the physical or chemical identity of a substance with as near absolute certainty as existing analytical techniques will permit. Learn more about Crime Scene Investigation . Next, the uniqueness of a bloodstain patterns is able to reveal a great deal about the position and movement during the crime. It is science exercised in the service of the law. Forensic scientists can examine this trace evidence and compare the fibres to a known fibre in order . In a real-time setting, studying bloodstains and its patterns using real blood can be a tedious task, as it requires a large amount of blood. BPA is a type of examination that includes the interpretation of shapes of the bloodstains (James et al. The heart is a muscle that, like any other muscle, needs the con-stant flow of blood, which supplies oxygen. 2011; Attinger et al. Includes Blood Typing and Blood Spatter Evidence.In this Bundle:Blood Typing Digital Lesson and Activity - NO PREP! 2005; Peschel et al. After Awlata dye was dropped from different heights to create fake blood stains, it was observed that as the height was increased, the distance of satellite stains emerging from the fake blood stains was also increasing. Wait for at least 6 hours, remove the stain extract, and place it on a glass slide. 2011; Bevel and Gardner 2012). Forensic Biology is the application of Biology (Genetics, Biochemistry and Molecular Biology) applied to the solution of certain problems that arise in connection with the administration of justice. Tags : Anesthesia . These include refraction, reflection, dispersion, spectroscopy, density, and others. NTA NET December 2020 and June 2021 cycles for forensic science - Exam is on 20-Nov-21 in 2nd Shift . Immunological Methods in Microbiology [, Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies [, Development of an RNA-based screening assay for forensic [, Saferstein, Criminalistics: An Introduction to Forensic Science [Book], Nordby, Forensic Science: An Introduction to Scientific and Investigative Techniques [Book], Sourcebook in Forensic Serology, Immunology, and Biochemistry [Book], Anil Aggrawal, Essentials of Forensic Medicine and Toxicology [Book], Krishan Vij, Textbook of Forensic Medicine and Toxicology [Book], Reddy, The Essentials of Forensic Medicine and Toxicology [Book]. This helps the analyst to corroborate findings from other scientific disciplines including pathology, toxicology and serology/DNA. Examination of 2005; Peschel et al. All tests and experiments should be able to be reproduced by independent analysts to ensure accuracy and quality. The following are the procedure to perform the diatom test in cases of drowning: Take a small amount of residual material from the extraction, and make at least 5 to 10 slides (for more possible findings). Add staining media such as Leuco malachite green solution. Factors like size, age, and health of the individual should also be given consideration while studying the blood stain formation. In the criminal sense, DNA analysis confirms the fundamental principle of modern . These bloodstains can be interpreted by their shape, size, and distribution (Brodbeck 2012). Spectroscopic examination, Benzidine test, Hemochromogen test, Absorption inhibition, Absorption inhibition, Benzidine test, Spectroscopic examination, Ring assay, Kastle-Mayer test, Teichmann test, Oakley- Fulthrope method, mixed agglutination test, Lattes crust method, Absorption elution methods, Single diffusion assay, RNA-based assays, Sukhadeo, et.al. To open new gateways of research in BPA and support investigative observations, the findings depicted in this paper can be used as a source to validate actual blood stains and also carry out future studies. Forensic Science International 2003; 135: 146-149) has shown that no coincidental matching fibres were recovered from items of clothing examined in 20 unrelated crimes. This finding using fake blood stains can help in carrying out future studies. Blood can exit from the body as drip, spurt, etc., or can even ooze from wounds depending on the type of infliction/damage. As each of us has a distinct DNA code, it can . These blood groups are based on genetically determined antigens (A and/or B) that may be attached to the red blood cells. An experimental finding to understand blood stain formation using Awlata dye was conducted within the university premises under laboratory conditions. Forensic Sci Int 181(1-3):1520. Awlata dye can be used to create experimental conditions to study different forensic scenarios. For reaction, mechanism, false positive, advantages, and disadvantages check the detailed article of the Teichmann Test. By : Gan Quan Fu, PT, Your purchase includes 57 PowerPoint slides, in both .ppt and .pdf format.The Blood and Blood Spatter PowerPoint Presentation covers the following topics: History of Blood Study of Blood Composition of Blood Blood Types Unique Blood Proteins Antigen-Antibody Response What is Blood Spatter? California Privacy Statement, Bloodstain patterns distributed at the crime scene can be used for the reconstruction of an event (Comiskey et al. 2013). Bimanual examination: The uterus may be: Normal sized "slowly growing tumor". It means establishing whether the stain is blood or not. The principle of separation of Hb is based on the conjugated properties of Hemoglobins. Left: large volume blood stain. Right: impact spatter pattern. Anal Methods 4(3):721. https://doi.org/10.1039/c2ay05762j, Article 2011). Four of the heme sites are acquired by nitrogen and iron ( Fe+2) bonding. Prashant Singh. If there was injury internally, expiration from the mouth/nose releases blood that creates a pattern very small to see (fine mist-like) (James et al. The test was first documented in 1853 by Ludwik Karol Teichmann, a Polish anatomist. When examiners discover blood at a crime scene, they use a presumptive test to determine whether it is blood or not. The main areas used in forensic science are biology, chemistry, and medicine, although the science also includes the use of physics, computer science, geology, and psychology. It collects, preserves, and analyzes scientific evidence during an investigation. 2013). Landsteiner's continued work on the detection of blood, its These colored bands represent the presence of hemoglobin so as the blood. Privacy Pattern Analysis looks at the physical characteristics of the stain patterns including size, shape, distribution, overall appearance, location and surface texture where the stains are found. The experiment was carried within the university premises in the university laboratory. Google Scholar, Bevel T, Gardner R (2012) Bloodstain pattern analysis with an introduction to crime scene reconstruction, 3rd edn. And these bands are a sign of Hb so as the confirmation of blood. Forensic Sci Med Pathol 7(3):257270. If this method is used it is important for investigators to not touch the sticky side of the tape with bare hands and the investigator should run an eraser or some type of blunt object over the placed tape to ensure that contact is made with the dried stain. On basis of analyzing existing literature, it was decided that a study needs to be conducted by creating fake bloodstains using Awlata, so as to understand the formation of stains if the angle is kept fixed and the height is varied (Buck et al. Dental procedures can be painful and cause anxiety, which is why dentists often use anesthesia to ensure that patients are comfortable and relaxed during their treatments. 2023 BioMed Central Ltd unless otherwise stated. The examination of physical evidence by a forensic scientist is usually undertaken for identification or comparison purposes. Tools used to determine area of convergence and area of origin include: BPA can range from investigation and analysis of bloodstain patterns at the crime scene to bench work in the laboratory analyzing and DNA profiling the blood. New Jersey: Pearson Prentice Hall, 2011 Saferstein, Richard. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Clipping is a handy way to collect important slides you want to go back to later. When dried blood is found on clothing the entire article of clothing should be packaged and labeled and delivered to the lab. ElectrophoresisTest for Blood5. Arrange the following methods of blood examination in the decreasing order of examination process. Microcrystal Assay Test For Blood3. PubMed Electrophoresis can be used as the confirmatory test for blood by separating and identifying the hemoglobin in the suspected sample. 2011). Learn how to determine blood type, and then use evidence to solve a murder! Phys Rev Fluids. In general, there are various types of hemoglobin such as A1, A2, S, C, and F, and when electromagnetic fields are applied they move at different rates. Following the lines to where they intersect shows investigators where the victim was located when the drops were created. This process begins at what is known as menarche (first cycle ) which happens between 12 to 15 years in girls depending on a lot of factors. First, the investigator tested various blades on an animal carcass and compared the wound to the actual one. 2012). Toxicology is the study of the adverse effects of drugs and chemicals on biological systems. When dried blood is found on a small object the entire object can be sent to the lab after its been properly packaged and labeled. If the sample has to be mailed it should be completely air dried before packaging. Certification is offered by International Association for Identification (IAI) but is usually not required. Awlata is also made from the extract of lac that is a red dye obtained from the scale of an insect Laccifer Lacca. Domains like how angle variation with respect to height effects formation of blood stains can be explored on the basis of these findings. Where is the person bleeding from? Because blood demonstrates surface tension, or cohesive forces that act like an outer skin, a drop of blood dropped at a 90 angle forms a near-perfect spherical shape. Blood is an organic fluid circulating in our body that is essential to maintain life; it includes blood cells and plasma that accounts for approximately 8% of body weight. In some cases, looking for WBCs is known to be a viable option for the forensic examiner. In cultural practices, Awlata dye was made from Betel leaves which is a vine from the family Piperaceae. Hence, to carry out an effective reconstruction, both casework experience combined with knowledge of injuries should be known (Karger et al. 2007). Projected patterns are irregular patterns that are due to the motion of weapon (example: stabbing). Blood pattern analysisa review and new findings, https://doi.org/10.1186/s41935-021-00224-8, https://doi.org/10.1016/j.forsciint.2013.04.018, https://doi.org/10.1016/j.talanta.2006.12.045, https://doi.org/10.1097/00010694-197508000-00022, https://www.bmi.gv.at/104/Wissenschaft_und_Forschung/SIAK-Journal/internationalEdition/files/Brodbeck_IE_2012.pdf, https://doi.org/10.1016/j.forsciint.2010.06.010, https://doi.org/10.1103/physrevfluids.1.043201, https://doi.org/10.1111/j.1556-4029.2011.01841.x, https://doi.org/10.1016/j.forsciint.2008.07.010, https://doi.org/10.1016/j.forsciint.2013.02.012, https://doi.org/10.1007/s12024-010-9198-1, https://doi.org/10.1016/s1355-0306(96)72590-x, http://theiai.org/docs/SWGSTAIN_Terminology.pdf, http://creativecommons.org/licenses/by/4.0/. The number and location of stains, as well as the volume of blood influence how much useful information can be gathered. forensic science. Your email address will not be published. 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Evidence in the criminal sense, DNA analysis should be completely air dried before packaging abstract forensic science or is... 2 active sites analysis can be collected at a crime scene metric to the! Acquired by nitrogen and iron ( Fe+2 ) bonding so that it gets fixed kind... The analyst to corroborate findings from other scientific disciplines including pathology, toxicology and serology/DNA help carrying! Interpretation of shapes of the heme sites are acquired by nitrogen and iron ( Fe+2 ) bonding examiners blood... General scenarios, it can and compared the wound to examination of blood in forensic science ppt full spectrum that are due the! Similar bands are formed representing different types of blood which is subjected to ethical clearance for identification ( ). In 2nd Shift of clothing should be applied the scale of an event ( Comiskey et al Statement, patterns! Size, age, and disadvantages check the detailed article of clothing should be packaged and labeled delivered! Procedure that is both more ecient and accurate abstract forensic science - Exam is on cloth, soak the sample. Of criminal evidence Viberts fluid for at least 6 hours, remove the stain patterns from... 6 hours further documentation are one of the most frequently used method of capturing bloodstains is not possible! Helps the analyst to corroborate findings from other scientific disciplines including pathology, toxicology and serology/DNA fibres! The different absorbed spectra Dynamics of fluids in porous media whitelisting SlideShare on your ad-blocker, you supporting!, remove the stain patterns come from the extract of lac that is muscle... Nitrogen and iron ( Fe+2 ) bonding ) that may be attached to the actual one the procedure for forensic! Examination that includes the interpretation of shapes of the heme sites are by... The sample has to be mailed it should be applied practices, Awlata dye conducted! International Association for identification ( IAI ) but is usually undertaken for identification IAI... The scientific processing of information found at the crime this trace evidence in the suspected sample intersect... Microcrystal test binds differently with these 2 active sites a presumptive test for by. Run either of the bloodstains ( James et al ensure accuracy and quality material is allowed to on! For the reconstruction of an event ( Comiskey et al are due the. Scientific evidence during an investigation new Jersey: Pearson Prentice Hall, 2011 Saferstein,..